Service Details

We offer a variety of single-molecule sequencing options on Nanopore's MinION or PromethION.

Plasmid-SIMPLE:

Our Plasmid-SIMPLE sequencing protocol includes transposon-based sequencing of an entire plasmid, no reference sequence required. This protocol requires 100-200ng of plasmid DNA as an input, and provides a minimum coverage of 100x. Customers will receive a fasta file with consensus, random positioning of the first base, and annotation from plAnnotate software. Original fastq files available upon request.

Plasmid-FS:

With higher sequencing depth and more detailed analysis, our Plasmid-FS protocol is perfect for challenging or unique plasmids, including long repeats and ITRs. Plasmid-FS orders include a unique cutter-based digestion and library preparation using Oxford-Nanopore's PCR-Free native barcoding kit. Sequencing coverage is 100x or up, as required by the client. 1-2 micrograms of plasmid input and a reference sequence are required for this protocol. Customers can expect to receive a consensus fasta file, a table of deviations from the reference, and a full basecall count with FRED quality sores. Original fastq files and BAM alignement are available upon request.

Other Nanopore Requests:

Our lab is equipped to handle a variety of different Nanopore applications and projects, including metagenomics, whole transcriptome, viral RNA sequencing, and variant screenings. We are happy to develop unique protocols to suit individual client's restrictions and preferences. Please reach out to us about your specific single-molecule sequencing needs.