Service Details

We provide Sanger Sequencing using ABI 3730xl machines. Sequencing reactions are typically run the same day our lab receives the sample. For customers utilizing our local pickup and delivery services, the lab will receive your samples the same day.

Clients may premix or send their own primers, but for partners who are comfortable sharing reference sequences with us, we order or select the appropriate primers for sequence from our inventory of 60,000+ oligos to reduce our partner's workload.

Customers may select from a sliding scale how to receive their data:

Raw Data

Clients receive AB1 chromatogram files and unedited FASTA files. Turnaround time within 12 hours.

Paired End Reads:

Clients receive AB1 chromatogram files and an edited, contiged FASTA file for each sample (2 reads per sample). Turnaround time within 24 hours except for bulk orders.

Fully Finished:

Fully Finished: Clients receive a full report on the sequencing results for each sample, including AB1 chromatogram files, edited and contiged FASTA files for each sample, and a description of any differences between the reference sequence and the sequencing data. Turnaround time depends on sample complexity.

Difficult Templates :

We specialize in difficult-to-sequence templates. Our protocols have been optimized for these difficult templates, and our team has decades of combined experience troubleshooting any problems that occur. Certain difficult templates may incur extra charges for our proprietary reagents; please contact us for more information.

• Inverted Terminal Repeats
• GC-Rich regions or homopolymers
• Poly-A/T Tails
• Di- and tri-nucleotide repeat stretches
• Long (> 1kb) direct repeats
• Long di-nucleotide stretches

Please let us know when you submit an order if any template contains any of these features so we can apply the appropriate protocol during sequencing.

Submission Guidelines:

We prefer samples submitted in 1.5ml microcentrifuge tubes. If sending 12+ samples, we prefer samples be sent in a 96-well plate and arranged by column. We can process samples in alternate formats, but occasionally result in sequencing delays. In general, plasmid samples require 250ng per 700 bases, and PCR samples require 25ng per 700 bases. When in doubt, send extra to avoid delays. For more detailed guidelines, please go here.

For more information on our Sanger Services, contact us.