Wyzer Biosciencess is specialized in NGS based on Illumina and Complete Genomics' SBS technology and Oxford Nanopore single-molecule sequencing. Our fleet of Illumina MiSeq and NextSeq instruments (Complete Genomics DNBSFQ-G99, Oxford Nanopre MinION and PromethION) allows us to perform rapid sequencing for:

• Targeted genes
• Small to large genomes
• Full Plasmids(PlasmidSimple™, Plasmid-FS%trade;)
• Amplicons
• Metagenomics samples
• Antibody screening

We use well-known DNA prep kits with option of Endotonxin-free. Our DNA transformation uses electrocompetent or chemically competent E coli cells. Sequencing confirmation for Midi to Giga preps are done with Nanopore. Sanger and restriction digestion analysis are available for additional charge. Starting material includes

• Colony
• Agar stab
• Culture
• Glycerol Stock
• Plasmid
• Colony

Our Plasmid-FS™ and Plasmid-Simple™ pipelines, which use Oxford-Nanopore Technologies sequencing, allow clients to obtain full-plasmid sequencing data faster and often at a lower price point.

To accelerate our customer’s projects, we provide a variety of adjacent molecular biology services, including DNA and RNA extraction, Normalization, Restriction Enzyme Digests, cDNA synthesis, PCR, and Exo-CIP™.

For partners with specific needs outside the scope of our current services, we are happy to work together to develop a custom pipeline. Please contact us directly with custom protocol requests..

Wyzer Biosciences provides the following finishing and confirmation services

• Basic Level
  • Manual error editing
  • Possible contig assembly if more than one read is performed for each sample
  • No detailed report is provided,only a general description.
• Advanced Level
• Error correction in assembled contigs and comparison to the provided references
• Possible contig assembly if more than one read is performed for each sample
• Custom design primers, apply specific sequencing chemistry to resolve potential issues caused by DNA quality/quantity.
• Provide detailed description of each sequencing sample, e.g. "Comparing to reference sequencing, sample has full double-stranded coverage between indicated regions without mutations"
• Sophisticated Level
• All services in Advanced level
• Vectors with two or more identical repeats longer than two good quality reads on an ABI instrument.
• Templates with >85% GC-content, long di- or tri-nucleotide repeats, AAV ITRs, mRNA long ploy A tail and long repeats.
• Single Molecule Confirmation (New for PlasmidSimple ™ and Plasmid-FS ™)
• Monitor various frequency mutations.
• Useful when combined with Level 3 service.